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AFLP Analysis

Amplified Fragment Length Polymorphism is a PCR based genetic fingerprinting technique developed by KeyGene. AFLP technology has the capability to detect various polymorphisms in different genomic regions simultaneously, is highly sensitive and reproducible and can generate large numbers of marker fragments for any organism, without prior knowledge of the genomic sequence. As a result, AFLP has become widely used for the identification of genetic variation in strains or closely related species of plants, fungi, animals, and bacteria; has been used in population genetics to determine slight differences within populations, and in linkage studies to generate maps for QTL analysis. GeneMarker software is compatible with output from Beckman CEQ™ , ABI PRISM®, or MegaBACE™ genetic analyzers; provides a faster, more user friendly analysis alternative to GeneMapper® for AFLP analysis and includes data normalization options. Unique sizing and pattern recognition technologies significantly improve analysis accuracy, requires less user intervention and provides greater analysis speed.

The software features high efficiency allele calling, adjustable parameters and various reporting options including a trace comparison report.

Comparison courtesy of Meghan Avolio,Yale University, Department of Ecology and Evolutionary Biology, New Haven CT 06520

GeneMarker’s New Large Fragment Sizing Technology expands and reduces the cost of the AFLP technique by affording never before multiplexing opportunities.

Allele Report displaying peak intensities

Report displaying presence and absence of alleles

Download AFLP Application Note
Download AFLP Comparative Analysis of Andropogon gerardii with GeneMarker® & GeneMapper® Software Packages

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Research use only (RUO)

GeneMarker Applications:
Fragile X
MLPA® (Multiplex Ligation‐dependent Probe Amplification)
MS‐MLPA®
Trisomy Analysis
Macromolecule Analysis Tool
ARMs & Comparative Analysis (Cystic Fibrosis Analysis)
MSI (Microsatellite Instability) Analysis
Merge Project
Kinship Analysis of Wild Populations and data base
AFLP
t‐RFLP
Microsatellite (SSR, STR, VNTR)
MLVA
SBE/ SnapShot®
Cluster Analysis
TILLING® & EcoTilling with slabgel or capillary data
SNPWave®/SNPlex®
Haplotype Analysis
Loss of Heterozygosity Analysis (LOH)









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