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SNPlex® & SNPWave® Analysis for Detection of Multiple Single Nucleotide Polymorphisms from DNA Fragment Analysis

One high‐throughput method to determine SNP genotypes is SNPWave (Keygene N.V.), which uses multiplex oligonucleotide ligation amplification of allele‐specific probes coupled with AFLP®‐primer selective amplification. Basic principle of SNPWave Multiplex allele-discrimination based on ligation using probes with unique stuffer (green). Circularizing padlock ligation probes are constructed that are specific to the SNP and flanking sequences. Locus‐specific probes hybridize to denatured genomic DNA. Allele‐specificity is determined by the SNP at the 5’ end of the padlock-probe. Probes that contain a 5’ nucleotide complementary to the SNP will be ligated and amplified by PCR in subsequent reactions. Alternatively, SNPlex Genotyping (Applied Biosystems) has been used to investigate SNPs in a diverse research, including breast cancer and to genotype plants. GeneMarker genotyping software is designed for fast, accurate and efficient analysis of SNPlex and SNPWave data from MegaBASE®, Beckman-Coulter® or ABI®PRISM capillary electrophoresis systems.

GeneMarker’s SNPlex Report

GeneMarker’s SNPWave Report

Download SNPlex Application Note
Download SNPWave Application Note

Trademarks property of their respective owners
Research use only (RUO)

GeneMarker Applications:
Fragile X
MLPA® (Multiplex Ligation‐dependent Probe Amplification)
MS‐MLPA®
Trisomy Analysis
Macromolecule Analysis Tool
ARMs & Comparative Analysis (Cystic Fibrosis Analysis)
MSI (Microsatellite Instability) Analysis
Merge Project
Kinship Analysis of Wild Populations and data base
AFLP
t‐RFLP
Microsatellite (SSR, STR, VNTR)
MLVA
SBE/ SnapShot®
Cluster Analysis
TILLING® & EcoTilling with slabgel or capillary data
SNPWave®/SNPlex®
Haplotype Analysis
Loss of Heterozygosity Analysis (LOH)









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