GeneMarker DNA Fragment Sizing Methods for Capillary Electrophoresis (CE) Data
As CE separation technology now achieves fragments in excess of 1000 bp, GeneMarker has a new sizing technology in addition to the traditional Local Southern, Cubic Spline and third order least squares for the middle fragments. These traditional methods provide highly accurate sizing for 50 – 600 bp, (ILS™600, ILS™500, GeneScan™HD400ROX™, GeneScan™500LIZ®-or 500ROX™, GeneScan™600, CEQ™400, CEQ™600, ET™400-R, ET™550-R) but have significant difficulties obtaining accurate size calls for data in excess of 800 bp, due to the nonlinear migration of larger DNA fragments.
Our “Large-Size” sizing technology provides accurate, linear sizing, one bp-resolution, using a DNA derivative migration time correction on fragments range from 30-1400bp (GeneScan™1200LIZ®) from ABI PRISM®, Beckman CEQ™ or MegaBACE™ genetic analyzers.
The “Large-Size” technology provides cost-reducing multiplexing opportunities, such as the addition of 4x-probes to clinical assays such as MLPA®, Allele Specific Amplification (ARMs™, ASA, OLA, etc.) forensic and ecology analysis including TILLING®, STR/microsatellite profiles, AFLP, T-RFLP, VNTR, ARISA, MLVA and BAC-fingerprinting.
Size Calibration Charts for review of Sizing Quality for all samples. Easily edit/update sizing calls for capillaries with decreased quality. Save or print the calibration charts for documentation of sizing quality for the project.
Download New Technology Size Calling application note
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MLPA® (Multiplex Ligation‐dependent Probe Amplification)
Macromolecule Analysis Tool
ARMs & Comparative Analysis (Cystic Fibrosis Analysis)
MSI (Microsatellite Instability) Analysis
Kinship Analysis of Wild Populations and data base
Microsatellite (SSR, STR, VNTR)
TILLING® & EcoTilling with slabgel or capillary data
Loss of Heterozygosity Analysis (LOH)