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Loss of Heterozygosity Analysis (LOH)

LOH occurs when a somatic cell contains only one copy of an allele due to non-disjunction during mitosis, segregation during recombination, or deletion of a chromosome segment. LOH becomes critical when the remaining allele contains a point mutation that renders the gene inactive. This is a common occurrence in cancers where a tumor suppressor gene is affected. GeneMarker fragment analysis software, compatible with all major genetic analyzers Beckman CEQ™ , ABI PRISM®, or MegaBACE™ , has been developed to aid researchers and technicians in the detection of LOH within cancer cells and is a user-friendly alternative to CEQ™ Fragment Analysis Software, MegaBACE ®Genetic Profiler, GeneMapper®, Genotyper® . Using a unique allele calling algorithm, GeneMarker uses the germ line reference to compare and detect LOH in patient samples and provides a customizable final report with electronic signature of analyst, patient/reference traces, ratio plots and a table of peak statistics.


Example of LOH Traces and Ratio Plots


Final LOH Report

Loss of Heterozygosity Detection with GeneMarker® (PDF)

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Research use only (RUO)

GeneMarker Applications:
Fragile X
MLPA® (Multiplex Ligation‐dependent Probe Amplification)
MS‐MLPA®
Trisomy Analysis
Macromolecule Analysis Tool
ARMs & Comparative Analysis (Cystic Fibrosis Analysis)
MSI (Microsatellite Instability) Analysis
Merge Project
Kinship Analysis of Wild Populations and data base
AFLP
t‐RFLP
Microsatellite (SSR, STR, VNTR)
MLVA
SBE/ SnapShot®
Cluster Analysis
TILLING® & EcoTilling with slabgel or capillary data
SNPWave®/SNPlex®
Haplotype Analysis
Loss of Heterozygosity Analysis (LOH)









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