Multiplex Ligation-dependent Probe Amplification (MLPA) is a rapid, high-throughput technique for detecting copy number changes, with both general and clinical research applications. Kits are commercially available through MRC Holland. GeneMarker provides an entire module dedicated to MLPA analysis. The MLPA analysis application is linked directly to the program, allowing the user to process raw data, normalize samples , and perform MLPA analysis all in one program - eliminating unnecessary data transfers.
MLPA analysis with GeneMarker is both rapid and accurate, allowing complete MLPA , MS-MLPA, or Luminex-MLPA analysis of 96 samples in just minutes. GeneMarker is compatible with the output from all major genetic analyzers, including: ABI®PRISM, Beckman-Coulter®, and MegaBACE®. Access MLPA panels through GeneMarker’s panel editor, our website, or firstname.lastname@example.org. GeneMarker’s MLPA application has been validated by research hospitals worldwide, is included in EuroGentest validation report, and is an excellent alternative to GeneMapper®, Genotyper™, GeneScan®, and Coffalyser.
Please keep reading to learn more about the benefits of MLPA analysis with GeneMarker.
Or click here to watch the MLPA analysis webinar.
Click here to read the MLPA application note.
MLPA Normalization Increases the Accuracy and Consistency of Analysis
GeneMarker gives users two methods of data normalization: Population normalization uses all probes in a sample to normalize the data (recommended for most chemistries) whereas Control Probe Normalization uses only the control probes to normalize (recommended when expecting numerous duplications and deletions). Both methods improve the linearity of the data, allowing more accurate and consistent results.
Automated Quality Control Increases Rigor and Efficiency
This feature is new to GeneMarker 2.6.0. The program automatically detects important quality control fragments within each sample, and alerts the user if the behavior of any fragment is outside of the norms recommended by MRC Holland. These settings help make quality control easier and more rigorous.
Detailed Reports Ensure Results are Communicated Clearly and Effectively
Reports include an informative header, electropherogram, ratio plot, validation box, and report table. They can be customized to display the entire sample, a particular peak, or clusters of peaks. Reports may be printed directly, or electronically saved as .PNG or .JPEG images.
Highlight Probes of Interest
Highlighting helps draw attention to probes of particular interest. These probes will also be displayed as triangles in the ratio plot. Highlighting may be included in the print reports as well.
Extensive Settings Allow Customization Of Analyses
Customize your analysis using GeneMarker's extensive settings. Or, jump in using the recommended defaults.
Use Templates to Save Settings For Commonly Used Chemistries
Analysis settings can be saved in templates, reducing clicks and increasing the speed of analysis.
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Ligation‐dependent Probe Amplification)
ARMs & Comparative
Analysis (Cystic Fibrosis Analysis)
of Wild Populations and data base
(SSR, STR, VNTR)
EcoTilling with slabgel or capillary data
Loss of Heterozygosity
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Research use only (RUO)