MLPA® Analysis

Multiplex Ligation-dependent Probe Amplification (MLPA) is a rapid, high-throughput technique for detecting copy number changes, with both general and clinical research applications. Kits are commercially available through MRC Holland. GeneMarker® software provides an entire module dedicated to MLPA analysis. The MLPA analysis application is linked directly to the program, allowing the user to  process raw data, normalize samples , and perform MLPA analysis all in one program - eliminating unnecessary data transfers.

MLPA analysis with GeneMarker software is both rapid and accurate, allowing complete MLPA , MS-MLPA, or Luminex-MLPA analysis of 96 samples in just minutes. GeneMarker software is compatible with the output from all major genetic analyzers, including: ABI®PRISM, Beckman-Coulter®, and MegaBACE®. Access MLPA panels through GeneMarker software's panel editor, our website, or GeneMarker software's MLPA application has been validated by research hospitals worldwide, is included in EuroGentest validation report, and is an excellent alternative to GeneMapper®, Genotyper™, GeneScan®, SequencePilot® and Coffalyser.Net Software.

Please keep reading to learn more about the benefits of MLPA analysis with GeneMarker software.
Or click here to watch the MLPA analysis webinar.
Click here to read the MLPA application note.

MLPA Normalization Increases the Accuracy and Consistency of Analysis

GeneMarker software gives users two methods of data normalization: Population normalization uses all probes in a sample to normalize the data (recommended for most chemistries) whereas Control Probe Normalization uses only the control probes to normalize (recommended when expecting numerous duplications and deletions). Both methods improve the linearity of the data, allowing more accurate and consistent results.

Automated Quality Control Increases Rigor and Efficiency

This feature is new to GeneMarker software 2.6.0. The program automatically detects important quality control fragments within each sample, and alerts the user if the behavior of any fragment is outside of the norms recommended by MRC Holland. These settings help make quality control easier and more rigorous.

Detailed Reports Ensure Results are Communicated Clearly and Effectively

Reports include an informative header, electropherogram, ratio plot, validation box, and report table. They can be customized to display the entire sample, a particular peak, or clusters of peaks. Reports may be printed directly, or electronically saved as .PNG or .JPEG images.

Highlight Probes of Interest

Highlighting helps draw attention to probes of particular interest. These probes will also be displayed as triangles in the ratio plot. Highlighting may be included in the print reports as well.

Extensive Settings Allow Customization Of Analyses

Customize your analysis using GeneMarker software's extensive settings; or, proceed directly to the analysis with the commonly used default settings.

Use Templates to Save Settings For Commonly Used Chemistries

Analysis settings can be saved in templates, reducing clicks and increasing the speed of analysis.

Share Panels, Size Standards, and Template Files Over Your Network

The working directories for panels, size standards, and templates may be changed, allowing these files to be easily updated, shared, and distributed over a network.

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