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MS-MLPAŽ Methylation-Specific-Multiplex Ligation-Dependent Probe Amplification for Genetic Disease and Genomic Imprinting Research

GeneMarker DNA analysis software is compatible with all major CE genetic-analyzers, an excellent alternative to GeneMapper®, Genotyper™, GeneScan®, CEQ™ Fragment Analysis Software for MLPA®, detecting genetic deletions and duplications in various diseases including cancer. Detection of methylated sites in genomic DNA is important 1) if the promoter is methylated, the gene will not be transcribed and its associated protein will not be produced; of great interest for cancer research and 2) DNA methylation is important in genomic imprinting (Prader-Willi Syndrome and Angelman Syndrome). Panels for analysis of data from Promoter Methylation kits from MRC‐Holland (including Tumor Suppressor, Mismatch Repair genes and genomic imprinting kits) are included with GeneMarker, easily downloaded or available from tech_support@softgenetics.com. GeneMarker’s new Methylation Specific – MLPA® module quickly and accurately detects methylation sites for researchers studying promoter methylation and genomic imprinting diseases. GeneMarker’s ease of use and professional reporting options are an excellent choice for MS‐MLPA® applications.

Methylation of the promoter region of tumor suppressor gene ESR1 is indicated by the red plot point that appears above the methylation threshold line.

The image on the left is a normal patient sample. Five genes
of interest (methylation specific) from the PWS/AS region of
chromosome 15 appear in the “Normal” region between the
methylation detection threshold lines. The image on the right
is a sample from a PWS patient. The five genes of interest
are in a 1:1 ratio with the reference trace indicating
methylation at these five sites (one copy of 15q11.2 and one
copy of 15q12 have been deleted).

Download MS-MLPA Application Note (PDF)

MLPA is a registered trademark of MRC-Holland B.V.

Trademarks property of their respective owners
Research use only (RUO)

GeneMarker Applications:
Fragile X
MLPA® (Multiplex Ligation‐dependent Probe Amplification)
MS‐MLPA®
Trisomy Analysis
Macromolecule Analysis Tool
ARMs & Comparative Analysis (Cystic Fibrosis Analysis)
MSI (Microsatellite Instability) Analysis
Merge Project
Kinship Analysis of Wild Populations and data base
AFLP
t‐RFLP
Microsatellite (SSR, STR, VNTR)
MLVA
SBE/ SnapShot®
Cluster Analysis
TILLING® & EcoTilling with slabgel or capillary data
SNPWave®/SNPlex®
Haplotype Analysis
Loss of Heterozygosity Analysis (LOH)









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