ARMs™ & Comparative Analysis (Application and Tools for Cystic Fibrosis DNA Fragment Analysis)
ARMS™ (Amplification Refractory Mutation System, Newton et al. 1989) allele specific amplification technology, detects point mutations, insertions or deletions in DNA. GeneMarker genotyping software accepts CE data from Beckman CEQ™, ABI PRISM®, or MegaBACE™ genetic analyzers providing fast, accurate genotyping; the linked ARMS/Comparative Analysis application compares file pairs for a given individual, presenting results in a concise clinical research report. It is ideal for analysis of samples from GenProbe® EU-2 for cystic fibrosis (or any project where the different dye channels for duplicate files need to be compared).
The Highlight Abnormal Allele Option in the main analysis window is ideal for CF analysis using kits such as the Abbott® Cystic Fibrosis Genotyping Assay (Oligonucleotide-Ligation-Assay, OLA). The main analysis screen provides rapid visual evaluation of mutant alleles in the electropherogram and Allele Report. Mutant allele labels are displayed in contrasting color in electropherogram for instant visualization of mutant alleles. MCC testing may be easily performed using ChimerMarker prior to prenatal genetic testing, for diseases
such as cystic fibrosis.
Review Results from GenProbe EU-2 in the ARMs application
Final Report includes Customized Header, Elecropherograms, Difference Histogram,
Report Tables and Authorization/Conclusion box.
Review Results from Abbott Cystic Fibrosis Genotyping Assay
Example of an allele report with a heterozygous mutations
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Ligation‐dependent Probe Amplification)
ARMs & Comparative
Analysis (Cystic Fibrosis Analysis)
of Wild Populations and data base
(SSR, STR, VNTR)
EcoTilling with slabgel or capillary data
Loss of Heterozygosity