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Detection and Quantification of Maternal Cell Contamination (MCC) in Chorionic
Villus (CV) or Amniotic Fluid (AV) used in Prenatal Testing for Genetic Disorders

Chorionic villus (CV) and amniotic fluid (AF) are common cell sources for prenatal molecular analysis of genetic disorders, such as aneuploidy/trisomy or cystic fibrosis. These samples may be contaminated with maternal tissue or maternal hematopoietic cells, decreasing the accuracy of genetic testing. The analyst must be confident that the sample used for analysis does not contain any maternal cell contamination. MCC analysis also serves as an internal quality assurance measure to ensure that the biological mother is matched with her concordant fetus(es); minimizing the possibility of sample mix-up. MCC analysis is a key requirement for umbilical cord blood banking amid concerns that maternal T cells may elicit GvHD post-UCB transplant. ChimerMarker software has a userfriendly interface, provides accurate genotyping of maternal and fetal samples and uses rigorous mixture analysis algorithms to automatically detect and quantify MCC. ChimerMarker is compatible with the output file format of all major capillary electrophoresis instruments (ABI®PRISM, Beckman-CEQ™, and MegaBACE®), human identity PCR kits (including Identiflier®, PowerPlex®16, PowerPlex®ESI, Profiler®) or custom chemistries. Contact info@softgenetics.com to request a free trial and online training.

Maternal Cell Contamination (MCC) Application in ChimerMarker Provides:
Accurate Size and Allele Calls
• Automated Detection and Quantification of MCC
• Save Time….Analyze Multiple Cases in One Project
• Create individual Case Reports – Quantification Follows MCC Best Practice Guideline (USA and European)
• Reduce Errors …. No data Transfer – Allele Calls and Linked MCC Quantification Application

Dialog Boxes to Easily Set MCC Analysis Paramters

Maternal Cell Contamination analysis dialog window contains parameters to quantify in area or height, ignore stutter peak, gender marker, statistics, and grouping options. Select Grouping to activate the File Group Editor.

The File Group Editor is used to group individual fetal samples to their respective maternal samples. After grouping of each case, the auto identify button can further classify each maternal and fetus sample into their respective category within each group.

MCC Results

By grouping and auto identifying each sample, the software can analyze each fetus sample by comparing the alleles to their respective maternal sample. Each individual patient or group is defined in the browser pane on the left. The middle pane shows all calculated results, genotypes and parameters. The electropherograms identify each allele in the fetus sample as either a fetus, maternal, or shared allele for easy reviewing.

Summary Report for Each Case with Custom Header and Authorization Box

ChimerMarker software’s final MCC report consists of a two-page summary. Page 1 contains the electropherograms with all alleles identified along with a customer header that contains both the analysis information, institution information, and sample information.

Page 2 contains the quantification, statistics, and genotypes detected in the fetus and maternal sample.

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Review Our Introductory and Double Donor Chimerism Analysis Webinars

Complete Chimerism Detection, Quantification and Analysis in one program:

Tools to Assist Analysts and Supervisors:

Application Notes:

ChimerMarker™ Software for Automated Analysis, Chimerism Detection, Quantification
and Monitoring from Short Tandem Repeat (STR) DNA in Post-Transplant Samples App Note (PDF)

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