Mutation Surveyor™ ... A
Unique Research Tool
Mutation Surveyor is available in 400 and 48 lane capacities.
Utilizing our patented anti-correlation technology, the program
rapidly locates all differences between the wild type sequence
and sample traces with excellent accuracy and sensitivity.
The program can be used with either single direction or bi-directional
Enhanced Indel Detection
Assembly and Alignment: SoftGenetics’ Robust Alignment
algorithm has been complemented by another product based algorithm.
The new technology utilizes a moving window throughout the
sequence trace continually matching the sample peaks to those
of the reference. When alignment ceases, program begins to “gap” either
sample or reference trace until alignment is restored.
Enhanced detection algorithm
allows routine detection of 1bp indels and
new alignment engine will correctly align traces
containing up to 10% variation from reference,
making them excellent tools for AIDS, plants
and other multi variant sequence traces.
Detection Sensitivity to 5%
Single as well as dual directional sequence traces are easily analyzed
for any potential variants using out patented anti-correlation technology which
performs an actual physical comparison of the sample/patient traces to the
reference traces. Found differences are indicated by the sharp peaks in the
Analysis courtesy of Dr. Yidong Bai, Assistant Professor,
University of Texas Health Center, San Antonio TX
Accuracy of the software in
the bi-directional analysis mode is over 99%,
with sensitivity to greater than 5% of the primary
Researchers no longer need to perform time consuming and inaccurate
comparison of the entire sample trace. With Mutation Surveyor,
any found variants of the sample when compared to the wild
type are clearly indicated in our mutation electropherogram
as a sharp peak.
Accuracy of the software in the bi-directional analysis mode
is over 99%, with sensitivity to greater than 5% of the primary
peak. Our collaborators have demonstrated an accuracy of 95%
when processing single direction sequence traces.
Mutation Surveyor’s detection sensitivity has been
enhanced to report similar peaks that are buried in baseline
noise of both the forward and reverse traces, alerting researchers
to the possible presence of mutations buried in the background
Mutation Surveyor easily processes 400 lanes of data in approximately
two minutes, and can be operated on a fully automated unattended
The software automatically forms contigs, performs alignments
and mutation detection comparing both forward and reverse patient
traces to reference or normal traces. Homozygote and Heterozygote
mutations are indicated by sharp peaks in our exclusive mutation
Unique algorithms perform a comparison of sample to reference
traces in both forward and reverse electropherograms checking
for similar peaks that are present in the background. This
technique provides almost analytical quality sensitivity.
click to enlarge
Homozygote Variants are detected when the color
of the reference drops 100% being replaced in the same spatial position
by a second color in the sample or patient.
click to enlarge
The software detects heterozygote
variants by observing a 50% drop in color of
reference, with a second wavelength growing in
approximately the same proportion and in the
same spatial position.
Insertions and deletions are found by monitoring the mobility
of the sample DNA fragments, again in comparison to the reference,
to that of the reference, providing an extremely low false
positive rate and ignoring the miscalls or overcalls from basecaller
software. In the event that a heterozygote insertion or deletion
is detected, the software will de-convolute the multiple patient
trace into two clean traces, while continuing with the mutation
click to enlarge
click to enlarge
heterozygote insertion /
Mutation Surveyor and
Explorer monitor the migration time of the DNA fragments
in comparison to the references. If the program detects
a change in the migration time, the center line turns
from green to red indicating the presence of an insertion
or deletion. The software then re-aligns the traces,
to calculate the Indel.
The position of the Indel is
indicated by the heavy red line.
Assembly of a “whole gene” mutational analysis
is easily accomplished with Mutation Surveyor. To assemble
the mutational analysis of an entire gene, simply enter the
Genbank data, pre-analyzed mutation projects, and the software
will assemble and calculate an allele frequency, providing
the locations of the found variants in bp order.
Reporting is extremely user-friendly with several customer
customizable reporting formats, or customer can easily cut
and paste areas of interest to any graphics capable program
such as Microsoft Word. Reports can be easily exported in text,
XML or HTML formats. Click
here to see examples of the reporting feature.
For additional information on the technology and operation of the program
please review our video manual.
Please contact us for pricing on
either the 48 or 400 lane capacity program.
Somatic Mutation Detection Technical Note [ pdf]