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Mutation Surveyor™ ... A Unique Research Tool

Mutation Surveyor is available in 400 and 48 lane capacities. Utilizing our patented anti-correlation technology, the program rapidly locates all differences between the wild type sequence and sample traces with excellent accuracy and sensitivity. The program can be used with either single direction or bi-directional data.

Analysis of Single and Bi-directional Data


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Single Direction Analysis

             


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Dual Directional Analysis

Enhanced Indel Detection

Assembly and Alignment: SoftGenetics’ Robust Alignment algorithm has been complemented by another product based algorithm. The new technology utilizes a moving window throughout the sequence trace continually matching the sample peaks to those of the reference. When alignment ceases, program begins to “gap” either sample or reference trace until alignment is restored.

Enhanced alignment engines allow routine detection of 1bp indels.

Enhanced detection algorithm allows routine detection of 1bp indels and multiple indels.

Surveyor/Explorer’s new alignment will correctly align traces containing up to 10% variation from reference, making them excellent tools for aids, plants and other multi variant sequence traces.

Mutation Surveyor/Explorer’s new alignment engine will correctly align traces containing up to 10% variation from reference, making them excellent tools for AIDS, plants and other multi variant sequence traces.

 

Detection Sensitivity to 5%

Single as well as dual directional sequence traces are easily analyzed for any potential variants using out patented anti-correlation technology which performs an actual physical comparison of the sample/patient traces to the reference traces. Found differences are indicated by the sharp peaks in the mutation electropherogram.

Analysis courtesy of Dr. Yidong Bai, Assistant Professor, University of Texas Health Center, San Antonio TX


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Accuracy of the software in the bi-directional analysis mode is over 99%,
with sensitivity to greater than 5% of the primary peak.

Researchers no longer need to perform time consuming and inaccurate comparison of the entire sample trace. With Mutation Surveyor, any found variants of the sample when compared to the wild type are clearly indicated in our mutation electropherogram as a sharp peak.

Accuracy of the software in the bi-directional analysis mode is over 99%, with sensitivity to greater than 5% of the primary peak. Our collaborators have demonstrated an accuracy of 95% when processing single direction sequence traces.

Mutation Surveyor’s detection sensitivity has been enhanced to report similar peaks that are buried in baseline noise of both the forward and reverse traces, alerting researchers to the possible presence of mutations buried in the background noise.

Mutation Surveyor easily processes 400 lanes of data in approximately two minutes, and can be operated on a fully automated unattended basis.

The software automatically forms contigs, performs alignments and mutation detection comparing both forward and reverse patient traces to reference or normal traces. Homozygote and Heterozygote mutations are indicated by sharp peaks in our exclusive mutation electropherogram.

Unique algorithms perform a comparison of sample to reference traces in both forward and reverse electropherograms checking for similar peaks that are present in the background. This technique provides almost analytical quality sensitivity.


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Homozygote Detection
Homozygote Variants are detected when the color of the reference drops 100% being replaced in the same spatial position by a second color in the sample or patient.

             


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Heterozygote Detection
The software detects heterozygote variants by observing a 50% drop in color of reference, with a second wavelength growing in approximately the same proportion and in the same spatial position.

Insertions and deletions are found by monitoring the mobility of the sample DNA fragments, again in comparison to the reference, to that of the reference, providing an extremely low false positive rate and ignoring the miscalls or overcalls from basecaller software. In the event that a heterozygote insertion or deletion is detected, the software will de-convolute the multiple patient trace into two clean traces, while continuing with the mutation detection.


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Dual Insertion

             


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heterozygote insertion / deletion

Mutation Surveyor and Explorer monitor the migration time of the DNA fragments in comparison to the references. If the program detects a change in the migration time, the center line turns from green to red indicating the presence of an insertion or deletion. The software then re-aligns the traces, to calculate the Indel.

The position of the Indel is indicated by the heavy red line.

Assembly of a “whole gene” mutational analysis is easily accomplished with Mutation Surveyor. To assemble the mutational analysis of an entire gene, simply enter the Genbank data, pre-analyzed mutation projects, and the software will assemble and calculate an allele frequency, providing the locations of the found variants in bp order.

Reporting is extremely user-friendly with several customer customizable reporting formats, or customer can easily cut and paste areas of interest to any graphics capable program such as Microsoft Word. Reports can be easily exported in text, XML or HTML formats. Click here to see examples of the reporting feature.

For additional information on the technology and operation of the program please review our video manual.

Please contact us for pricing on either the 48 or 400 lane capacity program.

Application Note

Somatic Mutation Detection Technical Note [ pdf]

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Phone: 814-237-9340 Fax: 814-237-9343 Email: info@softgenetics.com