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Recent Validations of Mutation Surveyor: Mutation Surveyor is available in 400, 48 and 24 lane capacities. Utilizing our patented anti-correlation technology, the program rapidly locates all differences between the wild type sequence and sample traces with excellent accuracy and sensitivity. The program can be used with either single direction or bi-directional data.
Enhanced Indel Detection Assembly and Alignment: SoftGenetics' Robust Alignment algorithm has been complemented by another product based algorithm. The new technology utilizes a moving window throughout the sequence trace continually matching the sample peaks to those of the reference. When alignment ceases, program begins to "gap" either sample or reference trace until alignment is restored.
Detection Sensitivity to 5%
Researchers no longer need to perform time consuming and inaccurate comparison of the entire sample trace. With Mutation Surveyor, any found variants of the sample when compared to the wild type are clearly indicated in our mutation electropherogram as a sharp peak. Accuracy of the software in the bi-directional analysis mode is over 99%, with sensitivity to greater than 5% of the primary peak. Our collaborators have demonstrated an accuracy of 95% when processing single direction sequence traces. Mutation Surveyor's detection sensitivity has been enhanced to report similar peaks that are buried in baseline noise of both the forward and reverse traces, alerting researchers to the possible presence of mutations buried in the background noise. Mutation Surveyor easily processes 400 lanes of data in approximately two minutes, and can be operated on a fully automated unattended basis. The software automatically forms contigs, performs alignments and mutation detection comparing both forward and reverse patient traces to reference or normal traces. Homozygote and Heterozygote mutations are indicated by sharp peaks in our exclusive mutation electropherogram. Unique algorithms perform a comparison of sample to reference traces in both forward and reverse electropherograms checking for similar peaks that are present in the background. This technique provides almost analytical quality sensitivity.
Insertions and deletions are found by monitoring the mobility of the sample DNA fragments, again in comparison to the reference, providing an extremely low false positive rate and ignoring the miscalls or overcalls from basecaller software. In the event that a heterozygote insertion or deletion is detected, the software will de-convolute the multiple patient trace into two clean traces, while continuing with the mutation detection.
Assembly of a "whole gene" mutational analysis is easily accomplished with Mutation Surveyor. To assemble the mutational analysis of an entire gene, simply enter the Genbank data, pre-analyzed mutation projects, and the software will assemble and calculate an allele frequency, providing the locations of the found variants in bp order. Reporting is extremely user-friendly with several customer customizable
reporting formats, or customer can easily cut and paste areas of interest
to any graphics capable program such as Microsoft Word. Reports can be
easily exported in text, XML or HTML formats. Click
here to see examples of the reporting feature. Application Note Somatic Mutation Detection Technical Note [pdf] Het Indel Application Note [pdf] UPMC Poster [pdf] Genetic Diversity Resequencing Analysis with Mutation Surveyor® [pdf]
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