SoftGenetics - Software PowerTools for Genetic Analysis





T-RFLP (Terminal-Restriction Fragment Length Polymorphism) Analysis for Microbial Diversity and ARISA (Automated Ribosomal Intergenic Spacer Analysis)

T-RFLP is a PCR based genetic fingerprinting technique for the study of microbial community structure based on variation in the 16S rRNA gene. These DNA fragments are commonly separated using capillary electrophoresis. One of the primers of a primer pair is labeled with fluorescent dye and used to amplify a selected region of a gene of interest by PCR. The resulting PCR fragment is digested with one (or more) restriction endonuclease(s) and the Terminal Restriction Fragments (T-RFs) are separated with an automated DNA analyzer (ABI PRISM®, Applied Biosystems® SeqStudio™ or Spectrum Compact CE Systems.). Microbial diversity in a community can be estimated by analyzing the number and peak heights of TRF patterns.


GeneMarker software has the ability to produce highly sensitive and reproducible results for T-RFLP analysis and Automated Ribosomal Intergenic Spacer Analysis (ARISA) by adjusting the AFLP Analysis default settings. Report tables may be printed or saved for import into other T-RFLP analysis software, such as T-REX.


GeneMarker® software's Large Fragment Sizing Technology expands and reduces the cost of these techniques by affording extended multiplexing opportunities.


T-RFLP Data Analysis

Figure 1: The peak table displayed below the sample electropherogram shows the option menu for editing items within the table. Clicking the right mouse button with the cursor on a highlighted cell will activate the editing menu.


GeneMarker Print Report Example

Figure 2: Comparison of electropherograms from four samples.


Review Application Note:
Download the application note: GeneMarker® Software for Terminal-Restriction Fragment Length Polymorphism (T-RFLP) Data Analysis (PDF)


MLPA® is the registered trademark of MRC Holland

SoftGenetics - Software PowerTools for Genetic Analysis

Software Applications

Site Tools



SoftGenetics - Software PowerTools for Genetic Analysis




Review all of our software products










SoftGenetics - Software PowerTools for Genetic Analysis




SoftGenetics Software PowerTools for Genetic Analysis provides current up-to-date information and pricing on all products.
All requests are secure.


SoftGenetics - Software PowerTools for Genetic Analysis




Request trial versions of our software and tap into our knowledge base



Trial Software

All of our software products are available for review and trial periods. Please use link to request a trial version.


Request Trial >>


Online Training

Our software professionals are available to you for online training of any of our products. Please contact an expert now.


Request Training >>



SoftGenetics provides online webinars to assist you in the growing knowledge of our products. Visit our Analysis Corner.


View Webinars >>


SoftGenetics - Software PowerTools for Genetic Analysis




Representatives are always ready to assist you

Name *
Email *

Message *


SoftGenetics - Software PowerTools for Genetic Analysis

100 Oakwood Avenue | Suite 350 | State College, PA 16803 | USA
Phone: 814-237-9340 or 888-791-1270


© 2020 SoftGenetics, LLC. All rights reserved

Trademarks are property of their respective owners

Research use only (RUO)


Web design by Lovett Creations