SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

 

SNPlex® & SNPWave® Analysis

One high-throughput method to determine SNP genotypes is SNPWave (Keygene N.V.), which uses multiplex oligonucleotide ligation amplification of allele-specific probes coupled with AFLP®-primer selective amplification. Basic principle of SNPWave Multiplex allele-discrimination based on ligation using probes with unique stuffer (green). Circularizing padlock ligation probes are constructed that are specific to the SNP and flanking sequences. Locus-specific probes hybridize to denatured genomic DNA. Allele‐specificity is determined by the SNP at the 5' end of the padlock-probe. Probes that contain a 5' nucleotide complementary to the SNP will be ligated and amplified by PCR in subsequent reactions. Alternatively, SNPlex Genotyping (Applied Biosystems) has been used to investigate SNPs in a diverse research, including breast cancer and to genotype plants. GeneMarker software genotyping software is designed for fast, accurate and efficient analysis of SNPlex and SNPWave data from MegaBACE®, Beckman-Coulter® or ABI®PRISM capillary electrophoresis systems.

 

SNPWave Anaylsis Display

 

Figure 1: The SNPlex report window displays a synthetic gel image, list of samples, cluster plot to analyze peak information and assign SNP genotypes, sample electropherogram and SNP genotyping report.

 

Download SNPlex Application Note
Download SNPWave Application Note

MLPA® is the registered trademark of MRC Holland

SoftGenetics - Software PowerTools for Genetic Analysis

Software Applications

Site Tools

 

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

SOFTWARE


Review all of our software products

 

 

 

 

 

 

 

 

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

 

SoftGenetics Software PowerTools for Genetic Analysis provides current up-to-date information and pricing on all products.
All requests are secure.

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

EXPERTISE


Request trial versions of our software and tap into our knowledge base

 

 

Trial Software

All of our software products are available for review and trial periods. Please use link to request a trial version.

 

Request Trial >>

 

Online Training

Our software professionals are available to you for online training of any of our products. Please contact an expert now.

 

Request Training >>

 

Webinars

SoftGenetics provides online webinars to assist you in the growing knowledge of our products. Visit our Analysis Corner.

 

View Webinars >>

 

SoftGenetics - Software PowerTools for Genetic Analysis

 

 

SEND US A MESSAGE


Representatives are always ready to assist you


Name *
Email *
Phone

###
-
###
-
####
Message *

 

SoftGenetics - Software PowerTools for Genetic Analysis

100 Oakwood Avenue | Suite 350 | State College, PA 16803 | USA
Phone: 814-237-9340 or 888-791-1270
Email: info@softgenetics.com

 

© 2020 SoftGenetics, LLC. All rights reserved

Trademarks are property of their respective owners

Research use only (RUO)

 

Web design by Lovett Creations