Variable | Definition |
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amount | The amount Variable plot scales all peak (signal) heights for a locus from a relative height to an absolute height. All expected peak heights therefore have real values (645, 992, 82, and so on) instead of relative values (0.5375, 0.8267, 0.0725, and so on). |
A | The Relative Locus Height Variable plot, organized by dye channel to ensure individual visibility, scales all the loci relative to a single locus, where there is one value for each marker. The value for one marker is set to 1.0 and the values for all other markers are set relative to this marker. Because some markers can be amplified more efficiently or less efficiently than other markers, the “A” variable allows for each marker to have a different peak height, given some number of copies of an allele and all other factors being equal. Note: No Degradation Variable plots are shown for the 1.0 marker; however, the associated statistical values and convergence value are detailed in the Stats & Convergence table that is displayed below the Degradation Variable plots. |
D | The Degradation Factor Variable plot scales down the peak (signal) heights of larger alleles, with a different amount of scaling per contributor. The D variable has one value per contributor, and in the calculations, linear or exponential degradation is dependent on molecular weight. |
mu_A | The mu_A Variable plot displays the average value of A, where “A” is the relative locus height. |